A field evaluation of the Coulter STKS.

作者: Brian A. Warner , David M. Reardon

DOI: 10.1093/AJCP/95.2.207

关键词: PathologyBlood preservationNuclear medicineLaser lightMean corpuscular volumeTeaching hospitalEdetate disodiumWbc differentialMedicine

摘要: The performance of the Coulter STKS (Coulter, Hialeah, FL) was evaluated in a busy computerized teaching hospital laboratory. compared with S Plus IV and manually performed 400 white blood-cell differentials. measured blood-count parameters (i.e., blood cells [WBCs], red [RBCs], hemoglobulin [Hb], mean corpuscular volume [MCV], platelets [PLTs]), very well between two aperture impedance-based systems; precision, linearity, lack carryover were excellent. WBC differential (DIFF), derived from combination impedance, conductance, laser light scatter, also precise; linear insignificant. DIFFs (n = 424) to manual differentials, r values 0.97, 0.73, 0.86 for neutrophils, lymphocytes, monocytes, eosinophils, respectively. DIFF Suspect Flagging system produced 6.2% false negatives 2.6% positives when technique. These further investigated discussed. stable 18 24 hours normal samples anticoagulated K2EDTA stored at 20 degrees C prior analysis. Storage same anticoagulant 4 immediate aspiration preserved analysis considerably longer than hours. characteristics make significant advancement automated hematology.

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