Production of a tributyltin-binding protein 2 knockout mutant strain of Japanese medaka, Oryzias latipes.
作者： Yoko Kato-Unoki , Yuki Takai , Yosuke Nagano , Satoshi Matsunaga , Shintaro Enoki
DOI: 10.1016/J.MARPOLBUL.2020.111601
关键词: Tributyltin 、 Oryzias 、 Gene expression 、 Binding protein 、 Function (biology) 、 Japanese Medaka 、 Lipocalin 、 Biology 、 Cell biology 、 Gene
摘要: Tributyltin-binding proteins (TBT-bps), members of the lipocalin family, bind TBT in fish blood and are presumed to contribute detoxification TBT. Recent studies have shown that many species TBT-bp genes, these genes induced by stresses such as exposure chemicals or pathogenic bacteria. However, function TBT-bps, mechanisms their induction activity still unclear. Here, towards elucidating functions TBT-bp2, we produced a TBT-bp2 knockout (TBT-bp2-/-) strain Japanese medaka, Oryzias latipes, using CRISPR/Cas9 system. Gene expression mutated was reduced, cDNA sequencing predicted protein structure suggested possible loss function. could be grown under normal conditions. Exposure TBT-bp2-/- medaka various future experiments is expected our understanding this novel system aquatic organisms.
elsevier.com
sci-hub.st 参考文章(30)
Günter P. Wagner, Koryu Kin, Vincent J. Lynch, Measurement of mRNA abundance using RNA-seq data: RPKM measure is inconsistent among samples. Theory in Biosciences. ,vol. 131, pp. 281- 285 ,(2012) , 10.1007/S12064-012-0162-3
Kei Nakayama, Naomi Sei, Yuji Oshima, Kosuke Tashiro, Yohei Shimasaki, Tsuneo Honjo, Alteration of gene expression profiles in the brain of Japanese medaka (Oryzias latipes) exposed to KC-400 or PCB126. Marine Pollution Bulletin. ,vol. 57, pp. 460- 466 ,(2008) , 10.1016/J.MARPOLBUL.2008.02.020
Gabriel Gutiérrez, Marı́a D. Ganfornina, Diego Sánchez, Evolution of the lipocalin family as inferred from a protein sequence phylogeny. Biochimica et Biophysica Acta. ,vol. 1482, pp. 35- 45 ,(2000) , 10.1016/S0167-4838(00)00151-5
Thierry Fournier, Najet Medjoubi-N, Dominique Porquet, Alpha-1-acid glycoprotein. Biochimica et Biophysica Acta. ,vol. 1482, pp. 157- 171 ,(2000) , 10.1016/S0167-4838(00)00153-9
Patrícia Nogueira, Joana Lourenço, Eleazar Rodriguez, Mário Pacheco, Conceição Santos, Jeanette M. Rotchell, Sónia Mendo, Transcript profiling and DNA damage in the European eel (Anguilla anguilla L.) exposed to 7,12-dimethylbenz[a]anthracene. Aquatic Toxicology. ,vol. 94, pp. 123- 130 ,(2009) , 10.1016/J.AQUATOX.2009.06.007
Yumi Oba, Akira Yamauchi, Yasuyuki Hashiguchi, Hina Satone, Shizuho Miki, Mohamed Nassef, Yohei Shimasaki, Takeshi Kitano, Miki Nakao, Shun-ichiro Kawabata, Tsuneo Honjo, Yuji Oshima, Purification and characterization of tributyltin-binding protein of tiger puffer, Takifugu rubripes. Comparative Biochemistry and Physiology C-toxicology & Pharmacology. ,vol. 153, pp. 17- 23 ,(2011) , 10.1016/J.CBPC.2010.07.009
Cem Kuscu, Sevki Arslan, Ritambhara Singh, Jeremy Thorpe, Mazhar Adli, Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease Nature Biotechnology. ,vol. 32, pp. 677- 683 ,(2014) , 10.1038/NBT.2916
Hina Satone, Yuji Oshima, Yohei Shimasaki, Takahiko Tawaratsumida, Yumi Oba, Eriko Takahashi, Takeshi Kitano, Shun-ichiro Kawabata, Yoshimitsu Kakuta, Tsuneo Honjo, Tributyltin-binding protein type 1 has a distinctive lipocalin-like structure and is involved in the excretion of tributyltin in Japanese flounder, Paralichthys olivaceus Aquatic Toxicology. ,vol. 90, pp. 292- 299 ,(2008) , 10.1016/J.AQUATOX.2008.08.019
Hina Satone, Jae Man Lee, Yumi Oba, Takahiro Kusakabe, Eriko Akahoshi, Shizuho Miki, Nobuo Suzuki, Yuichi Sasayama, Mohamed Nassef, Yohei Shimasaki, Shun-ichiro Kawabata, Tsuneo Honjo, Yuji Oshima, Tributyltin-binding protein type 1, a lipocalin, prevents inhibition of osteoblastic activity by tributyltin in fish scales. Aquatic Toxicology. ,vol. 103, pp. 79- 84 ,(2011) , 10.1016/J.AQUATOX.2011.02.009
Y. Hashiguchi, J. M. Lee, M. Shiraishi, S. Komatsu, S. Miki, Y. Shimasaki, N. Mochioka, T. Kusakabe, Y. Oshima, Characterization and evolutionary analysis of tributyltin-binding protein and pufferfish saxitoxin and tetrodotoxin-binding protein genes in toxic and nontoxic pufferfishes. Journal of Evolutionary Biology. ,vol. 28, pp. 1103- 1118 ,(2015) , 10.1111/JEB.12634