Targeted delivery of chemically modified anti-miR-221 to hepatocellular carcinoma with negatively charged liposomes
作者： Guangya Xiang , Taotao Zhou , Li Zhang , Fangqi Peng , Yifei Huang
DOI: 10.2147/IJN.S79598
关键词: Cancer research 、 Liposome 、 Receptor 、 Biology 、 Transferrin 、 PTEN 、 Gene silencing 、 In vitro 、 Hepatocellular carcinoma 、 Molecular biology 、 Genetic enhancement
摘要: Hepatocellular carcinoma (HCC) is one of the leading causes cancer-related death. Gene therapy was established as a new strategy for treating HCC. To explore potential delivery system to support gene HCC, negatively charged liposomal used deliver miR-221 antisense oligonucleotide (anti-miR-221) transferrin (Tf) receptor over expressed HepG2 cells. The liposome exhibited mean particle size 122.5 nm, zeta -15.74 mV, anti-miR-221 encapsulation efficiency 70%, and excellent colloidal stability at 4°C. Anti-miR-221-encapsulated Tf-targeted demonstrated 15-fold higher compared nontargeted in cells vitro. Anti-miR-221 effectively delivered cells, upregulated target genes PTEN, P27(kip1), TIMP3, greater silencing liposome. After intravenous injection into tumor-bearing xenografted mice with Cy3-labeled liposome, Cy3-anti-miR-221 successfully tumor site increased expressions TIMP3. Our results demonstrate that could be therapeutic modality human
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