Novel Detection of Insecticide Resistance Related P450 Genes and Transcriptome Analysis of the Hemimetabolous Pest Erthesina fullo (Thunberg) (Hemiptera: Heteroptera)
作者： Yang Liu , Haoyang Wu , Qiang Xie , Wenjun Bu
DOI: 10.1371/JOURNAL.PONE.0125970
关键词: Hemiptera 、 Genome evolution 、 Gene 、 GenBank 、 Gene expression profiling 、 Biology 、 Transcriptome 、 Genetics 、 18S ribosomal RNA 、 Sequence analysis 、 General Biochemistry, Genetics and Molecular Biology 、 General Agricultural and Biological Sciences 、 General Medicine
摘要: Erthesina fullo (Thunberg, 1783) is an economically important heteropteran species in China. Since only three nucleotide sequences of this (COI, 16S rRNA, and 18S rRNA) appear the GenBank database so far, no analysis molecular mechanisms underlying E. fullo’s resistance to insecticide environmental stress has been accomplished. We reported a de novo assembled annotated transcriptome for adult using Illumina sequence system. A total 53,359,458 clean reads 4.8 billion nucleotides (nt) were into 27,488 unigenes with average length 750 bp, which 17,743 (64.55%) annotated. In present study, we identified 88 putative cytochrome P450 analyzed evolution superfamilies, genes CYP3 clan related metabolizing xenobiotics plant natural compounds, fullo, increasing candidate P450. The sequenced greatly expands available genomic information could allow better understanding at systems biology level.
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