The expression of calcitonin receptor detected in malignant cells of the brain tumour glioblastoma multiforme and functional properties in the cell line A172.

摘要: Wookey P J, McLean C A, Hwang P, Furness S G B, Nguyen S, Kourakis Hare D L & Rosenfeld J V (2012) Histopathology 60, 895–910 The expression of calcitonin receptor detected in malignant cells the brain tumour glioblastoma multiforme and functional properties cell line A172 Aim:  Previous studies have indicated that (CTR) could be induced a proinflammatory environment. In present study, CTR-immunoreactivity (CTR-ir) was investigated tissue from patients with (GBM). Methods results:  immunohistochemical analysis GBM samples, tissues complex glomeruloid structures surrounded by were analysed for CTR-ir using anti-human CTR antibodies generated against two separate epitopes CTR. associated predominantly glial cells. Regions found 12 14 tumours (P < 0.05). Using confocal microscopy, identified also positive fibrillary acidic protein, nestin CD133. Antibodies verified immunoblots microscopy Cercopithecus aethiops(COS)-7 transfectants. Immunoblots membrane preparations CTR-positive lines demonstrated major band (∼67 kDa) minor (∼52 kDa), but intensity reversed A172. cultured A172 cells, stimulation adenylyl cyclase inhibition extracellular-regulated kinase (ERK)1/2 phosphorylation. Conclusions:  The findings (i) expressed glioma majority tested, (ii) CTR+/CD133+ (iii) second messenger systems functionally modified suggest might useful therapeutic target GBM.