HLA Typing for the Next Generation

摘要: Allele-level resolution data at primary HLA typing is the ideal for most histocompatibility testing laboratories. Many high-throughput molecular approaches are unable to determine phase of observed DNA sequence polymorphisms, leading ambiguous results. The use higher methods often restricted due cost and time limitations. Here we report on feasibility using Pacific Biosciences’ Single Molecule Real-Time (SMRT) sequencing technology high-resolution typing. Seven samples were typed HLA-A, -B -C. results showed that SMRT was able generate sequences spanned entire Class I genes allowed accurate allele calling. Eight novel genomic class identified, four alleles, three confirmed as extensions one corrected an existing reference sequence. This method has potential revolutionize field clinical impact achieving this level likely considerable, particularly in applications such organ blood stem cell transplantation where matching donors recipients their utmost importance.