Liquid chromatographic separation of aminoglycosides with pulsed amperometric detection
作者： John A. Polta , Dennic C. Johnson , Kurt E. Merkel
DOI: 10.1016/S0021-9673(01)81339-X
关键词: Reagent 、 Electrolyte 、 Polystyrene 、 Chromatography 、 Column chromatography 、 Sodium hydroxide 、 Chromatographic separation 、 Nebramycin 、 Chemistry 、 Amperometry
摘要: Abstract The absence of strong chromophoric groups in aminoglycoside antibiotics virtually eliminates the possibility their photometric detection analytical liquid chromatography Pulsed amperometric is described for sensitivity several nebramycin factors following separation on a neutral polystyrene column. alkaline eluent (0.25 M sodium hydroxide) appropriate as electrolyte electrochemical detection, and need post-column addition reagents avoided. use coupled fore-column illustrated on-line preconcentration when concentrations are below ca. 1 ppm 50-μl samples (i.e., 50 ng).
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sci-hub.se 参考文章(7)
Scott Hughes, Dennis C Johnson, Triple-pulse amperometric detection of carbohydrates after chromatographic separation Analytica Chimica Acta. ,vol. 149, pp. 1- 10 ,(1983) , 10.1016/S0003-2670(00)83155-4
Scott Hughes, P. Lawrence Meschi, Dennis C. Johnson, Amperometric detection of simple alcohols in aqueous solutions by application of a triple-pulse potential waveform at platinum electrodes Analytica Chimica Acta. ,vol. 132, pp. 1- 10 ,(1981) , 10.1016/S0003-2670(01)93871-1
Scott Hughes, Dennis C. Johnson, High-performance liquid chromatographic separation with triple-pulse amperometric detection of carbohydrates in beverages Journal of Agricultural and Food Chemistry. ,vol. 30, pp. 712- 714 ,(1982) , 10.1021/JF00112A022
J. A. Polta, D. C. Johnson, The Direct Electrochemical Detection of Amino Acids at a Platinum Electrode in an Alkaline Chromatographic Effluent Journal of Liquid Chromatography & Related Technologies. ,vol. 6, pp. 1727- 1743 ,(1983) , 10.1080/01483918308064887
D.S. Austin, J.A. Polta, T.Z. Polta, A.P.-C. Tang, T.D. Cabelka, D.C. Johnson, Electrocatalysis at platinum electrodes for anodic electoanalysis Journal of Electroanalytical Chemistry. ,vol. 168, pp. 227- 248 ,(1984) , 10.1016/0368-1874(84)87101-4
Scott Hughes, Dennis C. Johnson, Amperometric detection of simple carbohydrates at platinum electrodes in alkaline solutions by application of a triple-pulse potential waveform Analytica Chimica Acta. ,vol. 132, pp. 11- 22 ,(1981) , 10.1016/S0003-2670(01)93872-3
K. F. KOCH, K. E. MERKEL, S. C. O'CONNOR, J. L. OCCOLOWITZ, J. W. PASCHAL, D. E. DORMAN, STRUCTURES OF SOME OF THE MINOR AMINOGLYCOSIDE FACTORS OF THE NEBRAMYCIN FERMENTATION Journal of Organic Chemistry. ,vol. 43, pp. 1430- 1434 ,(1978) , 10.1002/CHIN.197834336