作者: Steven D. Douglas , Samuel K. Ackerman
DOI:
关键词: Baby hamster kidney cell 、 Immunology 、 Cell biology 、 Monocyte 、 Human blood 、 Chemistry
摘要: Human blood monocytes, but not other Ficoll-Hypaque cells, adhere to plastic surfaces from which confluent BHK cells have been removed. EDTA (3 mM) rapidly and completely removes providing a simple technique for preparation of human monocytes in suspension. The method should be valuable studies monocyte function where monolayers are suitable.