Purification of Human Monocytes on Microexudatecoated Surfaces

作者: Steven D. Douglas , Samuel K. Ackerman

DOI:

关键词: Baby hamster kidney cellImmunologyCell biologyMonocyteHuman bloodChemistry

摘要: Human blood monocytes, but not other Ficoll-Hypaque cells, adhere to plastic surfaces from which confluent BHK cells have been removed. EDTA (3 mM) rapidly and completely removes providing a simple technique for preparation of human monocytes in suspension. The method should be valuable studies monocyte function where monolayers are suitable.

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