sucker encodes a zebrafish Endothelin-1 required for ventral pharyngeal arch development.

摘要: Mutation of sucker (suc) disrupts development the lower jaw and other ventral cartilages in pharyngeal segments zebrafish head. Our sequencing, cosegregation rescue results indicate that suc encodes an Endothelin-1 (Et-1). Like mouse chick Et-1, suc/et-1 is expressed a central core arch paraxial mesoderm epithelia, both surface ectoderm endoderm, but not skeletogenic neural crest. Long before chondrogenesis, mutant embryos have severe defects crest expression dHAND, dlx2, msxE, gsc, dlx3 EphA3 anterior arches. Dorsal patterns are unaffected. Later development, display mesodermal endodermal tissues pharynx. Ventral premyogenic condensations fail to express myoD, which correlates with muscle defect. Further, shh endoderm first pouch fails extend as far laterally wild types. We use mosaic analyses show functions nonautonomously cells, thus required environment postmigratory cells specify fates. further mesendoderm for formation. Collectively our support model dorsoventral patterning gnathostome arches Et-1 cranial specifies