Thin-layer chromatographic detection of ivermectin in cattle dung.

作者: K.D Floate , W.G Taylor , R.W Spooner

DOI: 10.1016/S0378-4347(97)00153-9

关键词: Detection limitChromatographyAnthelminticChemistryFecesQuantitative analysis (chemistry)IvermectinThin layer chromatographicThin-layer chromatographyDerivatization

摘要: A qualitative method based on thin-layer chromatography (TLC) is described that reliably detects ivermectin as a fluorescent derivative in extracts of cattle dung. The limit detection (LOD) was < or = 40 ng/g wet These observations were statistically documented and shown visually using digital imagery. Residues detected fresh dung deposited by animals treated 10 days previously with topical dose (500 microg/kg body weight) ivermectin.

参考文章(30)
J. L. Bernal, M. J. Del Nozal, M. Salas, E. Galante, J. P. Lumaret, HPLC Determination of Residual Ivermectin in Cattle Dung Following Subcutaneous Injection Journal of Liquid Chromatography & Related Technologies. ,vol. 17, pp. 2429- 2444 ,(1994) , 10.1080/10826079408013491
L. Strong, T. A. Brown, Avermectins in insect control and biology: a review Bulletin of Entomological Research. ,vol. 77, pp. 357- 389 ,(1987) , 10.1017/S0007485300011846
Jan Fischer, Mary T. Kelly, Malcolm R. Smyth, Pavel Jandera, Determination of ivermectin in bovine plasma by column-switching LC using on-line solid-phase extraction and trace enrichment Journal of Pharmaceutical and Biomedical Analysis. ,vol. 11, pp. 217- 223 ,(1993) , 10.1016/0731-7085(93)80200-K
Patricia C. Tway, James S. Wood, George V. Downing, Determination of ivermectin in cattle and sheep tissues using high-performance liquid chromatography with fluorescence detection. Journal of Agricultural and Food Chemistry. ,vol. 29, pp. 1059- 1063 ,(1981) , 10.1021/JF00107A041
John W. Tolan, Philip Eskola, David W. Fink, Helmut Mrozik, Laurie A. Zimmerman, Determination of avermectins in plasma at nanogram levels using high-performance liquid chromatography with flourescence detection Journal of Chromatography A. ,vol. 190, pp. 367- 376 ,(1980) , 10.1016/S0021-9673(00)88241-2
L. Strong, Avermectins: a review of their impact on insects of cattle dung Bulletin of Entomological Research. ,vol. 82, pp. 265- 274 ,(1992) , 10.1017/S0007485300051816
H.T. Khor, Removal of chlorophyll pigments from plant neutral lipids Journal of Chromatography A. ,vol. 179, pp. 225- 226 ,(1979) , 10.1016/S0021-9673(00)80683-4
Richard Wall, Les Strong, Environmental consequences of treating cattle with the antiparasitic drug ivermectin Nature. ,vol. 327, pp. 418- 421 ,(1987) , 10.1038/327418A0
D.F. Cook, I.R. Dadour, D.N. Ali, Effect of diet on the excretion profile of ivermectin in cattle faeces International Journal for Parasitology. ,vol. 26, pp. 291- 295 ,(1996) , 10.1016/0020-7519(95)00132-8
W. Campbell, M. Fisher, E. Stapley, G Albers-Schonberg, T. Jacob, Ivermectin: A Potent New Antiparasitic Agent Science. ,vol. 221, pp. 823- 828 ,(1983) , 10.1126/SCIENCE.6308762