Arabidopsis Protein Microarrays for the High-Throughput Identification of Protein-Protein Interactions

摘要: Protein microarray technology has emerged as a powerful new approach for the study of thousands proteins simultaneously. microarrays have been used wide variety applications human and yeast systems. In recent study, we demonstrated that Arabidopsis functional protein can be generated employed to characterize function plant proteins. The arrayed were produced using an optimized large-scale plant-based expression system. proof-of concept 173 known novel potential substrates calmodulin (CaM) calmodulin-like (CML) identified in unbiased high-throughput manner. information documented here on CaM targets provides testable hypotheses area CaM/Ca2+-regulated processes represents resource scientific community.