Identification and characterization of genes and mutants for an N‐terminal acetyltransferase from yeast.

摘要: A gene from Saccharomyces cerevisiae has been mapped, cloned, sequenced and shown to encode a catalytic subunit of an N-terminal acetyltransferase. Regions this gene, NAT1, the chloramphenicol acetyltransferase genes bacteria have limited but significant homology. nat1 null mutant is viable exhibits variety phenotypes, including reduced activity, derepression silent mating type locus (HML) failure enter G0. All these phenotypes are identical those previously characterized mutant, ard1. NAT1 ARD1 distinct that proteins with no obvious similarity. Concomitant overexpression both in yeast causes 20-fold increase activity vitro, whereas either or alone does not raise over basal levels. functional iso-1-cytochrome c protein, which N-terminally acetylated strain, isogenic mutant. At least 20 other proteins, histone H2B, ard1 mutants. These results suggest function together catalyze acetylation subset proteins.