Microarray-basierte Analysen der genomischen Kopienzahl und der Genexpression beim Kolonkarzinom
作者： J. Gröne , H. J. Buhr , M. Hummel , D. Lenze
DOI: 10.1007/978-3-540-78833-1_7
关键词: Gene expression profiling 、 Gene expression 、 Chromosomal region 、 microRNA 、 Biology 、 Cancer 、 Cancer research 、 Single-nucleotide polymorphism 、 Colorectal cancer 、 Copy-number variation
摘要: Both, analysis of copy number variations (CNV) and gene expression profiling allow the identification pathological mechanism associated with tumor development progression. The aim study was to combine two technologies analyse a potential correlation chromosomal changes in colon cancer identify regions clinical impact. Gene single nucleotide polymorphism were collected from 32 stage UICC II patients after cell enrichment by macrodissection (Human Genome U133 Plus 2.0 Array Human Mapping 500K Set). Among several observed alterations we could show that region 13q31.3, comprising mikro-RNA Cluster 17–92 (C13orf25), is gained 22/32 (69 %) all samples (CNV(Med) 2,4). Comparison CNV differential this shows an overexpression C13orf25 tissue 17/ (foldchange (T vs. N) > 1,5). Patients amplification 13q31.3 showed 15 out 22 (Foldchange = 1,9) compared normal no C13orf25. Known members MYC-dependant microRNA cluster (miR-17–92) tissues be demonstrated cancer. Chromosomal synchronous relative miR-17–92 may hint for expression. Our strategy combining both allows molecular impact
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