The binding of cupric ions to bovine pancreatic ribonuclease studied with diligand metal-ion buffers

摘要: A procedure has been developed for the use of metal-ion buffers that depends on formation 2:1 complexes between suitable chelators and metal ions. beta-Alanine used as chelator Cu(2+) ions in a study binding by bovine pancreatic ribonuclease equilibrium-dialysis technique at pH7.0, 6.1 5.2. The results indicated presence two avid sites, more group being implicated inhibition enzyme activity constants site were 2.97x10(7), 7.97x10(5) 1.25x10(4) 5.2 respectively, less 5.27x10(6) 1.71x10(5) pH7.0 respectively. data show is chelated to protein through least ligand groups molecule.