The interaction of high mobility proteins HMG14 and 17 with nucleosomes

摘要: The interaction of the high mobility group proteins, HMG14 and HMG17, with nucleosome core particles has been studied. results show that two molecules HMG14/17 can be bound tightly but reversibly to each particle their affinity for is greater than histone-free DNA size. Thermal denaturation nuclease digestion studies suggest major sites are located near ends DNA. When preparations from chicken erythrocyte nuclei stripped HMG proteins partially titrated HMG14/17, nucleosome-HMG complex fraction enriched in beta-globin gene sequences.