Efficient transformation of mammalian cells with constructs containing a puromycin-resistance marker
作者： Susana de la Luna , Inmaculada Soria , Diego Pulido , Juan Ortín , Antonio Jiménez
DOI: 10.1016/0378-1119(88)90585-9
关键词: Puromycin 、 Biology 、 Selectable marker 、 Plasmid 、 Gene expression 、 Transformation (genetics) 、 Molecular biology 、 Genetic transfer 、 Recombinant DNA 、 Cell culture
摘要: Abstract Recombinant plasmids have been obtained that lead to the accumulation of five- ten-fold more puromycin- N -acetyl-transferase (PAC) mRNA and two- three-fold PAC activity than already described plasmid pSV 2 pac [Vara et al., Nucl. Acids Res. 14 (1986) 4117–4124]. When these optimized recombinants were used for stable transformation puromycin resistance, efficiencies up 1 × 10 −2 obtained, indicating -containing may be very useful dominant selectable markers gene transfer in mammalian cells.
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