Response to mercury (II) ions in Methylococcus capsulatus (Bath)
作者： Rich Boden , J. Colin Murrell
DOI: 10.1111/J.1574-6968.2011.02395.X
关键词: Methylococcus capsulatus 、 Biochemistry 、 Betaproteobacteria 、 Bacteria 、 Specific activity 、 Mercury (element) 、 Gammaproteobacteria 、 NAD+ kinase 、 Enzyme 、 Chemistry
摘要: The mercury (II) ion is toxic and usually detoxified in Bacteria by reduction to elemental mercury, which less toxic. This catalysed an NAD(P)H-dependent mercuric reductase (EC 1.16.1.1). Here, we present strong evidence that Methylococcus capsulatus (Bath) - a methanotrophic member of the Gammaproteobacteria uses this enzyme detoxify mercury. In radiorespirometry studies, it was found cells exposed dissimilated 100% [(14)C]methane provided generate reducing equivalents fuel reduction, rather than mix assimilation dissimilation control incubations. detoxification system constitutively expressed with specific activity 352 (+/- 18) nmol NADH oxidized min(-1) (mg protein)(-1). Putative genes were predicted M. genome mRNA microarray studies. MerA-derived polypeptide showed high identity (> 80%) MerA sequences from Betaproteobacteria.
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