Detection of cholera toxin gene in stool specimens by polymerase chain reaction: comparison with bead enzyme-linked immunosorbent assay and culture method for laboratory diagnosis of cholera.

摘要: Stool specimens obtained from 123 hospitalized patients with acute secretory diarrhea admitted to the Infectious Diseases Hospital, Calcutta, India, were examined for isolation of Vibrio cholerae O1 by direct or enrichment plating on selective media cholera toxin (CT) bead enzyme-linked immunosorbent assay (bead-ELISA) and CT gene polymerase chain reaction (PCR). V. was isolated either culture 70 stool specimens, all which gave positive results PCR. Eleven negative bead-ELISA also In addition, 13 more both method bead-ELISA, With combined a confirmed laboratory diagnosis could be made 81 while three methods, including PCR, yielded result 94 examined. From these data, we conclude that PCR provides sensitive specific rapid than currently available methods.