Techniques for Detecting Reactive Oxygen Species in Pulmonary Vasculature Redox Signaling

摘要: Redox signaling plays important roles in regulating pulmonary vasculature function. Aberrant redox signaling, e.g., overproduction of reactive oxygen species (ROS) that exceeds the capability cellular antioxidant mechanisms, has been found to alter function and remodel blood vessel structure, thus contributes pathological processes vasculature. The regulation via ROS is a very complicated process with various biological events involved, however, specific effect individual underlying mechanism still remain unclear. Most are present as free radical forms extremely short lifetime, which makes it difficult detect investigate their bioactivities. Therefore, developing sensitive methods complex system essential for us advance our knowledge regulation. In this chapter, we introduce several commonly used techniques detection vitro vivo, including chemiluminescence-based assay, fluorescence-based cytochrome c reduction method, genetically encoded fluorescent probes, well ESR spin trapping technique. We also discuss advantages, limitations, recent technical advances each technique applications studies. believe will provide better understanding on how maintain normal functions under oxidative stress.