Transglucosylation into stevioside by the enzyme system from Streptomyces sp.

摘要: The enzyme system from Streptomyces sp. W19–1 formed several kinds of transfer products (TPs) when incubated in the presence both stevioside (ST) and curdlan. Three major TPs (A, B, C) were separated purified using HP-20 column chromatography, gel filtration on TOYOPEARL HW-40F, preparative high-performance liquid chromatography.The structures three identified by chemical enzymatic methods; A is 13-O-β-sophorosyl-19-O-β-laminaribiosyl steviol, B 13-O-β-32-β-glucosylsophorosyl-19-O-β-glucosyl C 13-O-β-sophorosyl-19-O-β-laminaritriosyl steviol. obtained for first time a pure state.