Uptake and efflux of chloroquine by chloroquine-resistant Plasmodium falciparum clones recently isolated in Africa.

摘要: Abstract In recently isolated African Plasmodium falciparum clones, the intracellular chloroquine concentration at steady-state, under standard culture conditions, could not differentiate chloroquine-sensitive from resistant parasites. However, an atmosphere of air chloroquine-resistant P. clones released pre-accumulated [ 3 H]chloroquine more rapidly than sensitive clones. The very fast efflux drug (CQR) parasites resulted in a differential retained by and 2–3 times steady-state uptake appeared to be enhanced verapamil desipramine while opposite was observed with This confirmed suggestion that inhibits rapid CQR resulting readily detectable increase accumulation. These observations indicate biochemical phenotypes are similar those reported S.E. Asia Latin America consistent common molecular basis for phenomenon.