The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets.

摘要: A multifactor optimisation technique is successfully applied to develop a new HPLC method in which methyldopa, hydrochlorothiazide and amiloride were analysed determined on C18 column with detection at 286 nm. The optimal conditions of separation the aid response surface diagram — ‘window diagram’. effect simultaneously varying pH, proportion aqueous acetic acidum methanol mobile phase studied optimise separation. composition that provides an acceptable resolution short elution time water–methanol (75:25) pH 3.60. k’ values for after 1.40, 2.50 5.33, respectively. Relative retention (α) ratio hydrochlorothiazide/methyldopa amiloride/hydrochlorothiazide 1.767 2.159, Correlation coefficients calibration curves all analytes greater than 0.995 R.S.D. slope intercept respect linearity less 2%. quantitative analysis Alatan® tablets (Lek-Ljubljana). powdered are extracted methanol, containing caffeine as internal standard assayed by comparison peak areas liquid chromatography. high recovery (for about 100%) low (<2%) confirm good precision reproducibility chromatographic method.