Regulation of gene expression by insulin and tumor necrosis factor alpha in 3T3-L1 cells. Modulation of the transcription of genes encoding acyl-CoA synthetase and stearoyl-CoA desaturase-1.

摘要: Insulin and tumor necrosis factor alpha (TNF alpha) produce potent opposing physiological signals in adipocytes. However, genes that are co-regulated by the hormone cytokine during after adipocyte differentiation have not been characterized. Using 3T3-L1 cells, we studied regulation of expression encoding acyl-CoA synthetase (ACS), stearoyl CoA desaturase-1 (SCD-1), two enzymes play key roles metabolism long chain fatty acids. is required for triggering transcriptional activation ACS SCD-1 at an early stage differentiation. In mature adipocytes insulin elicits a 4-fold increase rates transcription genes. when treated with TNF causes 75-90% decrease levels mRNAs. The decline mRNA content associated similar decrements Thus, subject to stimulation counter-regulation (at level) alpha, respectively. effects observed developing terminally differentiated Unlike genes, encode lipogenic lipoprotein lipase malic enzyme level These observations on control suggest possible mechanisms which can markedly adjust their capacity acid response external stimuli.