The macrophage and HIV: basic concepts and methodologies.

摘要: Along with CD4+ T-lymphocytes, macrophage lineage cells serve as primary hosts for HIV replication in vivo. In some tissues such brain, where T-cell infection is essentially absent, the development of HIV-associated disease mediated through macrophages. This fact underscores importance experimental methods that yield results and conclusions accurately reflect mechanisms operational Unfortunately, our understanding key aspects HIV-macrophage interactions, most notably, features viral entry, replication, latency persistence, lags behind infection. While questions are best approached by direct examination patient specimens using immunohistochemistry phylogenetics, experiments based on macrophages vitro can, necessarily, identify elucidate events, molecular mechanisms, pathological consequences associated this addition, culture can provide isolation infectious from blood monocytes tissue macrophages, well subsequent continued propagation these isolates their host cell origin. Maintenance pedigree limits possibility alteration properties chemokine coreceptor usage may then no longer situation chapter focuses We describe cultivation human monocyte-derived maintenance, them. Also included a protocol accutase detachment. Accutase relatively new dissociation medium, used primarily stem research. laboratory, it has far out-performed all other providing gentle, yet thorough, detachment without need scraping, loss surface antigens or viability.