Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion
作者： U. Karsten , G. Papsdorf , G. Roloff , P. Stolley , H. Abel
DOI: 10.1016/0277-5379(85)90271-8
关键词: Electrofusion 、 Clone (B-cell biology) 、 Antibody 、 Biology 、 Monoclonal antibody 、 Molecular biology 、 Cytokeratin 、 Monoclonal 、 Cell fusion 、 Antigen 、 Oncology
摘要: Hybridomas producing mouse monoclonal antibodies to antigens of the human mammary carcinoma cell line, MCF-7, have been generated by electric field-mediated fusion at a frequency ten times higher than polyethylene glycol. One obtained recognizes cytoskeletal structure restricted epithelial cells and carcinomas with distribution pattern resembling cytokeratin 19.
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