Imaging of Oxygen Distribution in the Surface and Deep Areas of the Kidney

摘要: We have demonstrated previously that imaging of oxygen distribution based on the quenching phosphorescence is a highly useful tool for investigating delivery in tissues at microvascular level. The kidney offers an interesting model study because it receives disproportionate amount cardiac output relative to its contribution body weight. Moreover, unlike other tissues, bed contains both afferent and efferent arterioles within rich vascular network order control precisely filtration pressure glomerulus. Blood flow markedly heterogeneous, i.e., greater cortical regions than medullary ones, can be redistributed dependent upon volume state animal. Consequently, metabolic requirements areas may different accordance with capacity sodium retention. Finally, this organ sensing tissue linked production glycoprotein hormone, erythropoietin, during hypoxia. For these reasons, we initiated series experiments designed evaluate kidney. In particular, were interested potential effects endothelin (ET-1), since has been reported produce marked reductions renal blood (Badr et al., 1989) implicated pathogenesis acute ischemic injury (Kon 1989). Using images kinetic measurements pressures microvasculature kidney, preliminary data suggest low doses ET-1 rapidly decrease pressures.