Roles for CXC Chemokine Ligands 10 and 11 in Recruiting CD4+T Cells to HIV-1-Infected Monocyte-Derived Macrophages, Dendritic Cells, and Lymph Nodes

摘要: We investigated roles for chemoattractants in dissemination of HIV-1 by examining the induction T cell-active chemokines HIV-1-infected human monocyte-derived macrophages and dendritic cells. Of 12 analyzed, mRNAs two, CXCL10 CXCL11, ligands chemokine receptor CXCR3, were up-regulated both cell types upon infection HIV-1. Induction these genes infected cultures was dependent on viral entry reverse transcriptase activity, but not envelope glycoprotein. Conditioned medium from cells chemotactic freshly isolated CD4 + cells, chemotaxis abolished pretreatment with an Ab against CXCR3. A lymph node individual expressed CXCL11 paracortex, including venules, as detected situ hybridization, whereas neither mRNA after highly active antiretroviral therapy. Because CCR5 is found predominantly that also express data implicate recruitment susceptible to nodes, macrophages, This might enhance sequestration lymphoid organs spread between contributing immunopathology AIDS.