Use of a water-soluble fullerene derivative as precipitating reagent and matrix-assisted laser desorption/ionization matrix to selectively detect charged species in aqueous solutions.

摘要: A starlike water-soluble fullerene derivative, hexa(sulfonbutyl)fullerene (C60[(CH2)4SO3-]6; HSBF), consisting of a C60 cage covalently bonded with six negatively charged sulfonate arms, was synthesized and used to selectively precipitate positively surfactants, amino acids, peptides, proteins. The affinity HSBF the analytes depends on charge, structure, hydrophobic characteristics analytes. ion pair easily removed from solution by centrifugation. After washing, redissolved in solvent or buffer analyte characterized laser desorption ionization-time-of-flight mass spectrometry (LD-TOF). shows strong optical absorbance UV range, so no additional organic matrix required conduct LD-TOF analysis small For that contained five quaternary amines differing only alkyl chain length, exhibits highest amine longest chain. Only t...