Diversity of alpha-fetoprotein gene expression in mice is generated by a combination of separate enhancer elements

摘要: The 59 flanking region of the mouse alpha-fetoprotein (AFP) gene contains a tissue-specific promoter and three upstream regulatory elements that behave as classical enhancers. At least one these enhancers is now shown to be required for expression AFP when it introduced into genome by microinjection cloned DNA fragments fertilized eggs. Each enhancer can direct in appropriate tissues, visceral endoderm yolk sac, fetal liver, gastrointestinal tract, but each exerts different influence tissues. These differences may explain diversity levels characteristic gene. postnatal repression transcription both liver gut, well reinitiation its during regeneration, mimicked linked domains together or singly. Thus, sequence responsible directing activation transcription, repression, reinduction are contained limited segment within (or both) operative absence closely albumin