Profiling the main cell wall polysaccharides of tobacco leaves using high-throughput and fractionation techniques

摘要: Abstract Nicotiana species are used to study agriculturally and industrially relevant processes, but limited screening methods available for this species. A tobacco leaf cell wall preparation was fractionated using both chemical enzymatic methods; the fractions obtained were subsequently analysed rapid high-throughput profiling tools. The results confirmed previous data showing that mature walls predominantly composed of pectic homogalacturonans with lesser amounts hemicellulosic arabinoxyloglucan glucuronoxylan polymers. This confirmation provided proof could generate good-quality paves way mutants where a range biological profile is important, studied. novel oligosaccharide fingerprinting method optimized rapidly analyse structure XXGG-rich arabinoxyloglucans characteristic Solanaceae such as tobacco. Digestion profiles two xyloglucanase-specific endoglucanases: Trichoderma reseei EGII Paenibacillus sp. xyloglucanase compared latter enzyme has higher specificity toward arabinoxyloglucans, better-suited screening. methodology would be suitable species, tomato ( Solanum lycopersicum ) or potato tuberosum ), similar XXGG-type motifs in their xyloglucan structure.