Analysis of endogenous glutathione-adducts and their metabolites

摘要: The ability to conduct validated analyses of glutathione (GSH)-adducts and their metabolites is critically important in order establish whether they play a role cellular biochemical or pathophysiological processes. use stable isotope dilution (SID) methodology combination with liquid chromatography–tandem mass spectrometry (LC-MS/MS) provides the highest bioanalytical specificity possible for such analyses. Quantitative studies normally require high sensitivity that can be obtained by multiple reaction monitoring (MRM)/MS rather than much less sensitive but more specific full scanning methodology. method employs parent ion corresponding intact molecule together prominent product collision induced dissociation. Using SID LC-MRM/MS, analytes must have same relative LC retention time heavy internal standard established during validation procedure, correct ion. This level cannot attained any other technique employed biomarker analysis. review will describe application LC-MR/MS analysis GSH-adducts metabolites. It also discuss potential future directions this rigorous determination utility as disease exposure biomarkers.