In Vitro Cytotoxicity and Anti-inflammatory Cytokinine Activity Study of Three Isolated Novel Compounds of Prismatomeris glabra.

摘要: Objectives: The aim of the present study was to isolate and evaluate cytotoxicity anti-inflammatory activities new novel compounds isolated from Prismatomeris glabra. Materials Methods: Dried root P. glabra extracted under reflux with methyl alcohol, fractionated through vacuum liquid chromatography technique, evaporated then purified using column preparative thin-layer chromatography. THP-1 cells were treated amentoflavone, 5,7,4′-hydroxyflavonoid, stigmasterol various concentrations (0–30 µg/mL) incubated MTS reagent for 2h. Treatment done 24, 48, 72h. Then, effects these also tested on PGE2, TNF-α, IL-6 expression in human THP-1-derived macrophage 24h. Results: Three such as isolated. After 24h incubation, a significant decrease cell viability reported IC50 values 5,7,4′- hydroxyflavonoid, (21 µg/mL ≡ 38 M), (18 66 M) (20 48.5 respectively. Whereas 48 72h treatment showed less decreased compared treatment. These reduction production IL-6, PGE2 dose-dependent manner. Conclusions: effects.