P2X7-pannexin-1 and amyloid β-induced oxysterol input in human retinal cell: Role in age-related macular degeneration?
作者： Elodie Olivier , Mélody Dutot , Anne Regazzetti , Teddy Leguillier , Delphine Dargère
DOI: 10.1016/J.BIOCHI.2016.04.014
关键词: Signal transduction 、 Oxysterol 、 Toxicity 、 Retina 、 Receptor 、 Cancer research 、 Retinal 、 Macular degeneration 、 Biology 、 Pannexin 、 Internal medicine 、 Endocrinology
摘要: Age-related macular degeneration (AMD) is the most common cause of severe vision loss worldwide. Amyloid β involvement in degenerative diseases such as AMD well known and its toxicity has been related to P2X7 receptor-pannexin-1. Recently, oxysterols (oxidized derivatives cholesterol) have implicated pathogenesis. The aim our study was highlight amyloid β/oxysterols relationship describe receptor-pannexin-1 role toxicity. Using retinal epithelial cells, we first quantified sterols levels after incubation second investigated cytotoxic effects induced by oxysterols. For time, results showed that formation human pigmented cells. We oxysterol mediated receptor activation. This activation dependent on pannexin-1 with 25-hydroxycholesterol whereas signaling pathway pannexin-1-independent for 7-ketocholesterol. Taken together data suggest a pivotal cells which could be an important target develop new treatments AMD.
sci-hub.se 参考文章(54)
Joshua L Dunaief, John Q Trojanowski, Ann H Milam, Virginia M-Y Lee, Tzvete Dentchev, Amyloid-beta is found in drusen from some age-related macular degeneration retinas, but not in drusen from normal retinas. Molecular Vision. ,vol. 9, pp. 184- 190 ,(2003)
Juan A Orellana, Nicolas Froger, Pascal Ezan, Jean X Jiang, Michael VL Bennett, Christian C Naus, Christian Giaume, Juan C Sáez, None, ATP and glutamate released via astroglial connexin 43 hemichannels mediate neuronal death through activation of pannexin 1 hemichannels Journal of Neurochemistry. ,vol. 118, pp. 826- 840 ,(2011) , 10.1111/J.1471-4159.2011.07210.X
Kai Kaarniranta, Antero Salminen, Annakaisa Haapasalo, Hilkka Soininen, Mikko Hiltunen, Age-Related Macular Degeneration (AMD): Alzheimer's Disease in the Eye? Journal of Alzheimer's Disease. ,vol. 24, pp. 615- 631 ,(2011) , 10.3233/JAD-2011-101908
Björn Rissiek, Friedrich Haag, Olivier Boyer, Friedrich Koch-Nolte, Sahil Adriouch, P2X7 on Mouse T Cells: One Channel, Many Functions Frontiers in Immunology. ,vol. 6, pp. 204- 204 ,(2015) , 10.3389/FIMMU.2015.00204
Laura Malvitte, Thomas Montange, Anne Vejux, Corinne Joffre, Alain Bron, Catherine Creuzot-Garcher, Gérard Lizard, None, Activation of a Caspase-3-Independent Mode of Cell Death Associated with Lysosomal Destabilization in Cultured Human Retinal Pigment Epithelial Cells (ARPE-19) Exposed to 7β-Hydroxycholesterol Current Eye Research. ,vol. 33, pp. 769- 781 ,(2008) , 10.1080/02713680802337397
Anaïs Wakx, Mélody Dutot, France Massicot, Frédéric Mascarelli, G. Astrid Limb, Patrice Rat, Amyloid β Peptide Induces Apoptosis Through P2X7 Cell Death Receptor in Retinal Cells: Modulation by Marine Omega-3 Fatty Acid DHA and EPA. Applied Biochemistry and Biotechnology. ,vol. 178, pp. 368- 381 ,(2016) , 10.1007/S12010-015-1878-6
Thierry Pauloin, Mélody Dutot, Christophe Baudouin, Françoise Brignole-Baudouin, Hong Liang, Patrice Rat, Jean-Michel Warnet, Effects of toxic cellular stresses and divalent cations on the human P2X7 cell death receptor. Molecular Vision. ,vol. 14, pp. 889- 897 ,(2008)
Thierry Idziorek, Jérôme Estaquier, Frédéric De Bels, Jean-Claude Ameisen, YOPRO-1 permits cytofluorometric analysis of programmed cell death (apoptosis) without interfering with cell viability. Journal of Immunological Methods. ,vol. 185, pp. 249- 258 ,(1995) , 10.1016/0022-1759(95)00172-7
Jerzy Z. Nowak, Oxidative stress, polyunsaturated fatty acidsderived oxidation products and bisretinoids as potential inducers of CNS diseases: focus on age-related macular degeneration Pharmacological Reports. ,vol. 65, pp. 288- 304 ,(2013) , 10.1016/S1734-1140(13)71005-3
Pablo Pelegrin, Annmarie Surprenant, Pannexin-1 Couples to Maitotoxin- and Nigericin-induced Interleukin-1β Release through a Dye Uptake-independent Pathway Journal of Biological Chemistry. ,vol. 282, pp. 2386- 2394 ,(2007) , 10.1074/JBC.M610351200