Characterization of the Omega Class of Glutathione Transferases

摘要: The Omega class of cytosolic glutathione transferases was initially recognized by bioinformatic analysis human sequence databases, and orthologous sequences were subsequently discovered in mouse, rat, pig, Caenorhabditis elegans, Schistosoma mansoni, Drosophila melanogaster. In humans mice, two GSTO genes have been their genetic structures expression patterns identified. both species, GSTO1 mRNA is expressed liver heart as well a range other tissues. GSTO2 predominantly the testis, although moderate levels are seen Extensive immunohistochemistry rat tissue sections has demonstrated cellular subcellular specificity GSTO1-1. crystal structure recombinant GSTO1-1 determined, it adopts canonical GST fold. A cysteine residue place catalytic tyrosine or serine residues found GSTs shown to form mixed disulfide with glutathione. dehydroascorbate reductase thioltransferase activities also catalyze reduction monomethylarsonate, an intermediate pathway arsenic biotransformation. Other diverse actions include modulation ryanodine receptors interaction cytokine release inhibitory drugs. addition, linked age at onset Alzheimer's Parkinson's diseases. Several polymorphisms identified coding regions genes. Our laboratory GSTO2-2 proteins, number polymorphic variants. purification these proteins determination enzymatic activity described. Copyright 2005, Elsevier Inc. All rights reserved.