作者: Osamu Ishibashi , Mika Ikegame , Fumio Takizawa , Tatsuya Yoshizawa , Md. Ali Moksed
DOI: 10.1002/JCP.21968
关键词: Bone morphogenetic protein 、 Immunology 、 Endoglin 、 Cell biology 、 Periodontal fiber 、 Biology 、 Signal transduction 、 Population 、 SMAD 、 Bone morphogenetic protein 2 、 Cell culture
摘要: The periodontal ligament (PDL), a connective tissue located between the cementum of teeth and alveolar bone mandibula, plays crucial role in maintenance regeneration tissues. PDL contains fibroblastic cells heterogeneous cell population, from which we have established several lines previously. To analyze characteristics unique for at molecular level, performed cDNA microarray analysis versus MC3T3-E1 osteoblastic cells. followed by validation reverse transcription-polymerase chain reaction immunochemical staining revealed that endoglin, had been shown to associate with transforming growth factor (TGF)-β morphogenetic proteins (BMPs) as signaling modulators, was abundantly expressed but absent knockdown endoglin greatly suppressed BMP-2-induced differentiation subsequent mineralization. Interestingly, did not alter level Smad-1/5/8 phosphorylation induced BMP-2, while it expression Id1, representative BMP-responsive gene. Therefore, is conceivable regulates BMP-2-responsive genes some site downstream phosphorylation. Alternatively, found Smad-2 well phosphorylated BMP-2 cells, knockdown. These results, taken together, raise possibility respond via pathway dependent on involved mineralization J. Cell. Physiol. 222: 465–473, 2010. © 2009 Wiley-Liss, Inc.