Method to determine an antibody titre

作者: Rupert Dr Timpl , Jörgen Dr. Wieslander

DOI:

关键词: ImmunoassayAntibodyGlycosidic bondDisaccharideChemistryBiochemistryAlbuminChromatographyConjugateReceptorGalactose

摘要: To determine an antibody titre in human body fluids by the immunoassay principle incubation with at least two receptors R1 and R2, of which is present liquid phase carries a label, R2 mediates removal label bound to be determined, separation from unbound measurement one phases, used as or conjugate albumin disaccharide consists galactose units alpha -glycosidic linkage between them, where directly albumin, other receptor capable specific binding determined complex conjugate.

参考文章(2)
J�rgen Wieslander, Olle M�nsson, Elisabeth Kallin, Armando Gabreilli, Hans Nowack, Rupert Timpl, Specificity of human antibodies against gal?1-3gal carbohydrate epitope and distinction from natural antibodies reacting with gal?1-2gal or gal?1-4 gal Glycoconjugate Journal. ,vol. 7, pp. 85- 100 ,(1990) , 10.1007/BF01050405
H Towbin, G Rosenfelder, J Wieslander, J L Avila, M Rojas, A Szarfman, K Esser, H Nowack, R Timpl, Circulating antibodies to mouse laminin in Chagas disease, American cutaneous leishmaniasis, and normal individuals recognize terminal galactosyl(alpha 1-3)-galactose epitopes. Journal of Experimental Medicine. ,vol. 166, pp. 419- 432 ,(1987) , 10.1084/JEM.166.2.419