A rice‐derived recombinant human lactoferrin stimulates fibroblast proliferation, migration, and sustains cell survival

摘要: Human lactoferrin (hLF), a glycoprotein of the transferrin family, has recently been shown to stimulate wound repair through its antimicrobial effect and inflammation modulation. A recent study with several non-skin cell lines indicated that hLF may also have stimulatory on proliferation. To explore role in healing, we used recombinant human (holo-rhLF), derived from transgenic rice, examine effects holo-rhLF proliferation, migration, attachment, survival primary skin fibroblast culture system. This revealed not only significantly stimulates proliferation but synergistic growth factor-2 antagonistic transforming factor-β1 Furthermore, using chamber migration assay, our results demonstrate promotes dosage-dependent manner. More importantly, increased viability protected cells death when they were stressed by either serum depletion or 12-O-tetradecanoylphorbol-13-acetate exposure. No significant was observed attachment. In conclusion, these findings reveal multiple functions fibroblasts indicate potential application therapy enhancing as well protecting apoptosis.