MicroRNAs Are Aberrantly Expressed in Hypertrophic Heart : Do They Play a Role in Cardiac Hypertrophy?

摘要: MicroRNAs (miRNAs) are a recently discovered class of endogenous, small, noncoding RNAs that regulate gene expression. Although miRNAs highly expressed in the heart, their roles heart diseases currently unclear. Using microarray analysis designed to detect majority mammalian identified thus far, we demonstrated aberrantly hypertrophic mouse hearts. The time course aberrant miRNA expression was further hearts at 7, 14, and 21 days after aortic banding. Nineteen most significantly dysregulated were confirmed by Northern blot and/or real-time polymerase chain reaction, which miR-21 striking because its more than fourfold increase when compared with sham surgical group. Similar up-regulated miRNA, miR-21, also found cultured neonatal cardiomyocytes stimulated angiotensin II or phenylephrine. Modulating via antisense-mediated depletion (knockdown) had significant negative effect on cardiomyocyte hypertrophy. results suggest involved cardiac hypertrophy formation. might be new therapeutic target for cardiovascular involving such as hypertension, ischemic disease, valvular diseases, endocrine disorders.