Enzymatic characterization and functional analysis of EcChi3C from ridgetail white prawn Exopalaemon carinicauda.
作者： Yuying Sun , Jiquan Zhang , Fengge Song , Jing Wang , Zhenzhen Zhang
DOI: 10.1016/J.IJBIOMAC.2017.12.103
关键词: Crustacean 、 Ultrastructure 、 Gene 、 Hepatopancreas 、 Chitinase 、 Uropod 、 Biochemistry 、 Prawn 、 Moulting 、 Biology
摘要: Abstract Chitinase belongs to the glycosyl hydrolases family 18 and plays key role in development pathogen resistance of crustaceans. In this study, enzymatic characterization chitinase 3C (EcChi3C) Exopalaemon carinicauda was analyzed. addition, we analyzed expression profiles EcChi3C at different tissues molting stages. all tested tissues, it predominantly expressed hepatopancreas, then stomach, but poor other tissues. periods, mainly intermolt stages, The results molting, mortality uropod ultrastructure prawns after being injected with dsRNA were accordance those control group. there is no difference for endopodite morphology between survival dead individuals experimental After challenged bacteria, up-regulated significantly 12 h followed a comeback 96 h. These suggest that an important immune related gene not necessary process E. carinicauda.
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