Antigen-Specific CD4+ T Cells Produce Sufficient IFN-γ to Mediate Robust Protective Immunity against Genital Chlamydia muridarum Infection

作者: Weidang Li , Ashlesh K. Murthy , M. Neal Guentzel , J. Seshu , Thomas G. Forsthuber

DOI: 10.4049/JIMMUNOL.180.5.3375

关键词: MHC class IIBacterial vaccineChlamydia muridarumAntigen presentationAdoptive cell transferImmunologyInterferon gammaBiologyVaccinationImmunity

摘要: Chlamydia has been shown to evade host-specific IFN-gamma-mediated bacterial killing; however, IFN-gamma-deficient mice exhibit suboptimal late phase vaginal muridarum clearance, greater dissemination, and oviduct pathology. These findings introduce constraints in understanding results from murine chlamydial vaccination studies context of potential implications humans. In this study, we used deficient either IFN-gamma or the receptor for intranasal with a defined secreted Ag, protease-like activity factor (CPAF), plus CpG examined role derived adoptively transferred Ag-specific CD4+ T cells protective immunity against genital C. infection. We found that early induction cell infiltration correlates onset clearance. Adoptively competent CPAF-specific failed enhance resolution infection within recipient receptor-deficient mice. Conversely, production was sufficient induce reduction subsequent provide first direct evidence enhanced anti-C. induced by is dependent upon signaling such produce mediate effects. Additionally, MHC class II pathway robust immunity. Thus, targeting soluble candidate Ags via may be viable vaccine strategy optimal effective human

参考文章(42)
Becca Asquith, Christophe Debacq, Arnaud Florins, Nicolas Gillet, Teresa Sanchez-Alcaraz, Angelina Mosley, Luc Willems, Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester (CFSE). Proceedings of The Royal Society B: Biological Sciences. ,vol. 273, pp. 1165- 1171 ,(2006) , 10.1098/RSPB.2005.3432
Robert C. Brunham, Babak Pourbohloul, Sunny Mak, Rick White, Michael L. Rekart, The unexpected impact of a Chlamydia trachomatis infection control program on susceptibility to reinfection. The Journal of Infectious Diseases. ,vol. 192, pp. 1836- 1844 ,(2005) , 10.1086/497341
Sandra G. Morrison, Hua Su, Harlan D. Caldwell, Richard P. Morrison, Immunity to murine Chlamydia trachomatis genital tract reinfection involves B cells and CD4(+) T cells but not CD8(+) T cells. Infection and Immunity. ,vol. 68, pp. 6979- 6987 ,(2000) , 10.1128/IAI.68.12.6979-6987.2000
Feng Dong, Youmin Zhong, Bernard Arulanandam, Guangming Zhong, Production of a Proteolytically Active Protein, Chlamydial Protease/Proteasome-Like Activity Factor, by Five Different Chlamydia Species Infection and Immunity. ,vol. 73, pp. 1868- 1872 ,(2005) , 10.1128/IAI.73.3.1868-1872.2005
Jyotika Sharma, Youmin Zhong, Feng Dong, Jeanna M. Piper, Guqi Wang, Guangming Zhong, Profiling of Human Antibody Responses to Chlamydia trachomatis Urogenital Tract Infection Using Microplates Arrayed with 156 Chlamydial Fusion Proteins Infection and Immunity. ,vol. 74, pp. 1490- 1499 ,(2006) , 10.1128/IAI.74.3.1490-1499.2006
Feng Dong, Mustak Pirbhai, Youmin Zhong, Guangming Zhong, Cleavage-dependent activation of a chlamydia-secreted protease. Molecular Microbiology. ,vol. 52, pp. 1487- 1494 ,(2004) , 10.1111/J.1365-2958.2004.04072.X
James I. Ito, Joseph M. Lyons, Role of gamma interferon in controlling murine chlamydial genital tract infection. Infection and Immunity. ,vol. 67, pp. 5518- 5521 ,(1999) , 10.1128/IAI.67.10.5518-5521.1999