作者: Weidang Li , Ashlesh K. Murthy , M. Neal Guentzel , J. Seshu , Thomas G. Forsthuber
DOI: 10.4049/JIMMUNOL.180.5.3375
关键词: MHC class II 、 Bacterial vaccine 、 Chlamydia muridarum 、 Antigen presentation 、 Adoptive cell transfer 、 Immunology 、 Interferon gamma 、 Biology 、 Vaccination 、 Immunity
摘要: Chlamydia has been shown to evade host-specific IFN-gamma-mediated bacterial killing; however, IFN-gamma-deficient mice exhibit suboptimal late phase vaginal muridarum clearance, greater dissemination, and oviduct pathology. These findings introduce constraints in understanding results from murine chlamydial vaccination studies context of potential implications humans. In this study, we used deficient either IFN-gamma or the receptor for intranasal with a defined secreted Ag, protease-like activity factor (CPAF), plus CpG examined role derived adoptively transferred Ag-specific CD4+ T cells protective immunity against genital C. infection. We found that early induction cell infiltration correlates onset clearance. Adoptively competent CPAF-specific failed enhance resolution infection within recipient receptor-deficient mice. Conversely, production was sufficient induce reduction subsequent provide first direct evidence enhanced anti-C. induced by is dependent upon signaling such produce mediate effects. Additionally, MHC class II pathway robust immunity. Thus, targeting soluble candidate Ags via may be viable vaccine strategy optimal effective human