Применение электроаналитических методов для характеристики клеток человека

摘要: Rapid development of cell technologies stipulates the need for informative and express methods analysis viability physiological activity human cells. We studied analytical possibilities novel tools comprehensive characterization bioelectrochemical properties living cells, e.g. surface charge cellular membrane redox metabolites. Using Malvern Zetasizer dynamic light scattering analyzer we proposed an approach to assessment zeta potential cells detection phosphatidylserine on their as early apoptotic marker. On basis modified electrodes designed sensors exhibiting high sensitivity towards electroactive metabolites including antioxidants macroergic molecules. The were applied metabolic activity/energetic status (blood cultures). Electrochemical signal adenine nucleotides sensor correlated with intracellular level ATP according luciferase assay was found be more sensitive alteration in than conventional MTS test. disposable screenprinted fabricated a prototype portable rapid health (e.g. donor cells) less 5 ƒl volume sample. Proposed are interest transplantology, basic research cell-based medical diagnostics.