Comparison of pause predictions of two sequence-dependent transcription models

摘要: Two recent theoretical models, Bai et al (2004, 2007) and Tadigotla (2006), formulated thermodynamic explanations of sequence-dependent transcription pausing by RNA polymerase (RNAP). The two models differ in some basic assumptions therefore make different yet overlapping predictions for pause locations, on kinetics mechanisms. Here we present a comprehensive comparison the models. We show that while they have comparable predictive power locations at low NTP concentrations, model is more accurate than higher concentrations. predicted also consistent with time-course reactions, unsuited this type kinetic prediction. More importantly, general predict mechanisms even same sites, provides an explanation single molecule observations.