Effect of Carbon Source on Datura metel Microspore Embryogenesis and the Growth of Callus Raised from Microspore-derived Embryos

摘要: Summary The effects of various carbohydrates (fructose, glucose, sorbose, lactose, maltose, mannose, sucrose, mannitol, sorbitol and glycerol) on microspore embryogenesis the growth embryoderived callus Datura metel were studied. Among tested, mannitol promoted androgenic response, whereas, fructose, maltose mannose inhibited it. On lactose containing medium, anther response was not affected significantly, but majority embryos, developed this failed to grow beyond globular or heart-shaped stage. Sorbose only carbohydrate which completely response. most these similar what is being recorded for plantlet yield cultured anthers. sugars supporting appreciable callus, sucrose best, followed by fructose maltose. In addition, slight growth, in terms increase either fresh dry weight, also observed media glycerol, sorbose sorbitol. However, proved be inhibitory.