Upregulation of the vascular endothelial growth factor, Flt-1, in rat hippocampal neurons after envenoming by Phoneutria nigriventer; age-related modulation.

摘要: This study characterizes the distribution and quantifies expression of tyrosine kinase receptor for vascular endothelial growth factor (VEGF), Flt-1, in rat hippocampus following intra-peritoneal injection Phoneutria nigriventer venom (PNV). Post-natal day 14 (P14) 8–10 weeks (adult) old rats were used analyses done at 1, 2, 5 24 h after exposure compared with saline-injected counterparts. PNV-injected animals showed hippocampal venules perivascular edema indicating blood–brain barrier (BBB) dysfunction. was accompanied by significant overexpression Flt-1 which though not same CA1, CA2, CA3 dentate gyrus (DG) regions, neither P14 adult rats. Regional analysis using GIMP methodology that constitutively distributed more densely neurons DG, followed CA1/CA2 both control animals, without variation over time, but significantly expressed than adults. A time-course upregulation progressive VEGFR1/Flt-1+ PNV-exposed are timely regionally modulated depending on region, being CA2 least responsive region regardless animal's age, whilst DG most susceptible having higher neonates. Since VEGF has been reported to confer protection several pathological processes we suggest may be involved response via mediation PNV envenoming; its envenomed an indication neuroprotective is efficient age. The incidence young indicate a pro-inflammatory role receptor.