Removal of nonhomologous DNA ends in double-strand break recombination: the role of the yeast ultraviolet repair gene RAD1.

摘要: Double-strand breaks (DSBs) in Saccharomyces cerevisiae can be repaired by gene conversions or deletions resulting from single-strand annealing between direct repeats of homologous sequences. Although rad1 mutants are resistant to x-rays and complete DSB-mediated mating-type switching, they could not recombination when the ends break contained approximately 60 base pairs nonhomology. Recombination was restored were made donor Additionally, absence RAD1 led frequent appearance a previously unobserved type product. These data suggest is required remove nonhomologous DNA 39 recombining DNA, process analogous excision photodimers during repair ultraviolet-damaged DNA.