Plasma collected from heparinized blood is not suitable for HCV-RNA detection by conventional RT-PCR assay

摘要: Detection of hepatitis C virus-RNA (HCV-RNA) in serum or plasma is considered a reliable marker for ongoing HCV infection. HCV-RNA was measured routinely collected from heparinized blood patients with chronic and varying results were found. In this study, detected peripheral mononuclear cells (PBMCs) 16 out 17 anti-HCV positive C. contrast, found only one these seventeen patients. The addition heparin to known containing solutions before reverse-transcription separately PCR, demonstrated the inhibitory effect on reverse transcription amplification reaction. Furthermore, evidence provided that PCR should be preferably into EDTA vacutainers. It concluded, therefore, not suitable determination by RT-PCR. addition, PBMCs might play an important role pathobiology virus, since easily PBMC