Contribution of molecular analysis to the typification of the non-functioning pituitary adenomas.

作者: Laura Sanchez-Tejada , Ruth Sanchez-Ortiga , Cristina Lamas , Rosa Camara , Pedro Riesgo

DOI: 10.1371/JOURNAL.PONE.0180039

关键词: Thyroid-stimulating hormoneSomatotropic cellPathologyHousekeeping genePituitary glandCorticotropic cellGene expression profilingContext (language use)MedicineImmunohistochemistry

摘要: Aim The WHO Classification of Tumours Endocrine Organs considers the inmunohistochemical characterization pituitary adenomas (PA) as mandatory for patient diagnosis. Recent advances in knowledge molecular patterns these tumours could complement this classification with gene expression profiling. Methods Within context Spanish Molecular Registry Pituitary Adenomas (REMAH), a multicentre clinical-basic research project, we analysed phenotype 142 PAs complete IHC and clinical information. Gene levels all hormones, type 1 corticotrophin-releasing hormone receptor, dopamine receptors arginine vasopressin receptor 1b were measured by quantitative real-time polymerase chain reaction. In addition, used three housekeeping genes normalization pool nine healthy glands from autopsies calibration reference standard. Results Based on clinically functioning PA (FPA: somatotroph, corticotroph, thyrotroph lactotroph adenomas), established interquartile range relative studied each subtype. That allowed molecularly different subtypes, including non-functioning (NFPA). Afterwards, estimated concordance immunohistochemical diagnosis FPA between them NFPA. The kappa values higher than showed bad NFPA subtypes. Conclusions According to results, complements analysis, allowing better typification

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