Removal of lipids from human plasma low-density lipoprotein by detergents
作者： Kai Simons , Ari Helenius
DOI: 10.1021/BI00789A019
关键词: Low-density lipoprotein 、 Very low-density lipoprotein 、 Low-density lipoprotein receptor-related protein 8 、 Intermediate-density lipoprotein 、 Immunodiffusion 、 Ultracentrifuge 、 Dialysis (biochemistry) 、 Human plasma 、 Chromatography 、 Chemistry
摘要: Ari Helenius and Kai Simons t abstract: Four different detergents have been found to remove all major lipids from human plasma low-density lipoprotein (LDL). The detergents used were a bile salt-sodium deoxycholate, a neutral detergent-Nonidet P40, an anionic detergent-sodium dodecyl sulfate, and a cationic detergent-cetyltrimethylammonium bromide. High con-centrations of these detergents were added to aqueous solution of LDL. The protein moiety could then be separated from cV characterization of the lipid-free protein moiety (apo …
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