Extraction of nucleic acids from agarose gels
作者： J. Langridge , P. Langridge , P.L. Bergquist
DOI: 10.1016/0003-2697(80)90266-3
关键词: Spin column-based nucleic acid purification 、 Nucleic acid 、 Sepharose 、 Agarose 、 Aqueous two-phase system 、 Nucleic acid quantitation 、 Nucleic acid thermodynamics 、 Chromatography 、 Chemistry 、 Gel electrophoresis of nucleic acids
摘要: Abstract A rapid, simple and versatile method is described for the extraction from agarose gels of small plasmid molecules DNA fragments generated by restriction endonucleases. The may be used also RNA agarose-urea gels. It based on partitioning nucleic acid into 1-butanol as their quaternary ammonium salts, leaving neutral in aqueous phase. then recovered sodium salt partition back an Nucleic samples were found to unaffected treatment, judged ability ligated, transformed, nick-translated, vitro protein-synthesizing system.
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