Suitability of mapped sequence tagged microsatellite site markers for establishing distinctness, uniformity and stability in aromatic rice

摘要: At present, testing for distinctness, uniformity and stability (DUS) of crop varieties relies on a set morphological characters. These characters suffer fromthe limitations number, interaction with the environment in which variety grows subjectivity decision-making. The potential DNA-based markers such as sequence tagged microsatellite site (STMS), establishing DUS merits investigation. In present study, 55 mapped STMS markers, selected from 12 linkage groups rice genome, was used to examine distinctness 23 aromatic genotypes including commercially important Basmati varieties. Forty-one these (74.5%) showed polymorphism between number alleles per locus ranged 2–4 an average 2.3. information content (PIC) varied 0.083 0.665 0.338. All could be differentiated each other at low probability (0.07×10-13) identical match by chance. marker-based clustering corresponded known phenotypic classification, thereby providing confidence established markers. utility study analysed using crossbred Pusa 1(IET-10364) that contributes about 40–50% export India. Genotyping twenty individual plants, grown nucleus, breeder, foundation, certified farmer's saved seed samples all revealed no variation among plants. observations suggested employed this further studying commercial 1.